| The Sidi M'Hamed Ben Taïba dam is located near the town of Arib. It is used both for drinking water supply and for irrigation. The objective of the study which done in two locations : Sidi M’Hamed Ben Taïba dam and Water Treatment Plant of Arib is to enhance the physicochemical and bacteriological quality of water. As well as, to evaluate the biodegradation capacities of the microbial strains isolated from the water of the Sidi M' Hamed Ben Taïba dam. Our work consists first of all in determining the physicochemical parameters of the raw water of the Sidi M'Hamed Ben Taïba dam on three levels (surface, 15 m and 30 m) mainly: temperature, pH, nitrate, nitrite, organic matter, etc. Then the bacteriological quality by accounting total coliforms, faecal coliforms, faecal streptococci and sulphite-reducing Clostridium. In the second part, we based on the biodegradation of an oil sample on solid medium Mineral Salts Medium by the use of microorganisms isolated from the water of the dam. Twenty-seven cells grouped into five genera: Pseudomonas, Staphylococcus, Vibrio, Stenotrophomonas and Chryseobacter are identified. The stumps assimilate oil as a carbon source giving degradation zones of different diameters. Pseudomonas flurescens species showed significant degradative activity among other isolated bacterial species.
Keywords: Sidi M'Hamed Ben Taïba Dam, Water Treatment Plant, Arib, Physicochemical parameters, Bacteriological quality, Water, Oil, MSM, Biodegradation.
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