| Bioremediation techniques, which use naturally occurring microorganisms to eliminate hazardous organic chemical residues and contaminated areas, are constantly evolving. Actinomycetes have gained importance as they play a significant role in the recycling of organic matter and the production of novel pharmaceuticals, cosmetics, enzymes, antitumor agents, enzyme inhibitors, immune modifiers, and vitamins. Diverse Actinomycetes genera have the potential to be utilized in the bioconversion of underutilized urban and agricultural waste into high-value chemical compounds. This study was conducted to isolate actinomycetes capable of producing waste-degrading enzymes from floral waste vermicompost. In this study, floral waste was decomposed using the technique of vermicomposting. The floral wastes and cow dung in a ratio of 1:1 (50% each) were fed to Eisenia fetida earthworms for two months. Twenty actinomycetes were isolated and characterized morphologically using the
soil dilution technique on starch-casein agar media. The amylase, protease, and peptonization-coagulation activities were determined through a screening procedure. Almost every isolating organism demonstrated maximum enzyme production. Actinomycetes growth patterns and mycelial coloration were documented. The cultural and morphological analyses identified the actinomycetes genera as Streptomyces.
Keywords: Floral Waste, Ecofriendly waste treatment, Actinomycetes, Enzyme production
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