2.Study on hepatic histopathological observations and the ameliorative effects of Ocimum sanctum on mancozeb induced toxicity in albino mice |
S. Razia and A. Siddiqui |
The protective effects of Ocimum sanctum Linn was studied on liver of albino mice induced by different doses of mancozeb. Three groups of Swiss albino mice were used wherein distilled water at 0.1ml per mice per day orally was used in group I as control while the same volume of mancozeb dissolved in distilled water at 4.2mg/kg/bw and 6.4 mg/kg/bw were administered consecutively for 6 days a week for 4 weeks in group II and III respectively as the treated groups. Aqueous Ocimum sanctum extract was given at 400mg/kg/bw after withdrawal of mancozeb for 4 weeks in group II and III in the same way. There was significant decrease in the liver weight. The histopathology of the liver tissues of mancozeb treated group revealed impairment of normal structural organisation. The hepatic lobules and cord-like arrangements of liver cells were lost. Severe congestion was seen in central and portal vein. In high dose of mancozeb necrosis and cytoplasmic vacuolisation was evident along with pyknotic nuclei. Inflammatory leucocytic infiltrations were also observed. Exposure to Ocimum sanctum treatment reduced the lesions and healing was seen with better development in low dose of mancozeb (group I). No cytoplasmic vacuolisation was seen in the hepatocytes and most cells appeared binucleated displaying certain degree of recovery. The results of the present work indicate the ameliorative effect of aqueous extract of Ocimum sanctum against hepatic damage induced by mancozeb on albino mice. |