2.Growth determination of agriculturally important fungi of Black Cotton Soil rhizosphere of Soybean in Ujjain region
D. M. Kumawat, Rajesh Tiwari and Manish K Sharma
Abstract: Black cotton soil is characteristics of Malwa region (Madhya Pradesh) and covers Ujjain district. This soil type is rich in nutrients as well as diverse microbial population. This region cultivates Soybean as main kharif crop with different high yielding, resistant varieties suitable for climatic and edaphic conditions. Present study was undertaken to isolate and identify the rhizospheric fungi of JS–335 cultivar of soybean grown in the Black Cotton Soil. After identification selected fungi (Trichoderma harzianum, Aspergillus niger ANRT1 and Aspergillus niger ANRT2) were tested their growth response against environmental conditions such as temperature (15, 20, 25, 30, and 35°C) pH (5, 6, 7, 8, and 9) and carbon source (Glucose, Sucrose, Lactose, Maltose and Mannitol). Fungal growth was determined by radial growth on agar medium plates while biomass was determined by centrifugation dry–weight method. All data were tested statistically. Results showed that out of three studied fungi, Trichoderma harzianum grow best at temperature 25°C and showed maximum radial growth and biomass production, while 6 pH was suitable for all the three fungal isolates. Glucose was found best among all five, as all three fungi showed maximum growth in terms of radial growth and biomass. Present study emphasizes and proves that optimum conditions are very necessary for the growth and activity of agriculturally important fungi. Study also suggests that environmental conditions should be checked before and after crop seed sowing and cultivation so that these fungi can work efficiently and ultimately increase the yield of the crop. Keywords: Fungi, Agriculturally Important Microorganisms (fungi).